Snail transfection을 이용한 누드마우스에서의 침윤성 종양 형성
Xenografted Tumorigenesis in the oral vestibule of nude mice by Snail transfection: Histological and immunohistochemical study
김문기, 이은하, 김진, 육종인, 차인호,
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김문기 ( Kim Moon-Key ) - 국민건강보험공단 일산병원 구강악안면외과
이은하 ( Lee Eun-Ha ) - 국민건강보험관리공단 일산병원
김진 ( Kim Jin ) - 국민건강보험관리공단 일산병원
육종인 ( Yook Jong-In ) - 국민건강보험관리공단 일산병원
차인호 ( Cha In-Ho ) - 국민건강보험관리공단 일산병원
KMID : 0355620090350040199
Abstract
Purpose: The purpose of this study is to investigate the epithelial-mesenchymal transition (EMT) induced by Snail transcription factor and Snailtransfected in vivo tumors with histopathological features.
Materials and Methods : We induced in vivo xenografted tumorigenesis in the oral vestibules of nude mice by a Snail transfected HaCaT cell line and investigated morphological and immunohistochemical features in Snail expressive tumors.
Results: We identified tumor masses in 14 out of 15 nude mice in the HaCaT-Snail cell inoculation group, but no tumors were present in any of the HaCaT cell inoculation group. Induced tumors showed features of poorly differentiated carcinoma with invasion to neighboring muscles and bones. The HaCaT-Snail tumors showed decreased expressions of E-cadherin and cytokeratin, but showed increased expressions of vimentin and N-cadherin.
Discussion: The Snail transfected xenograft can improve productivity of malignant tumors, show various histopathological features including invasive growth, and aid in the investigation of tumor progression and the interaction with surrounding tissues.
키워드
E-cadherin;neoplastic cell transformation;Snail transcription factor;xenograft model
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